Injection:Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re- ligation with T4 DNA ligase (ThermoScientific) to generate pAAV- GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232- PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5- HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV- GFP to generate pAAV- SERT.
Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5-HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Article Title: Analyzing the neuroglial brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484 , Alias: 5-HTT, AI323329 , Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Transduction:Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re- ligation with T4 DNA ligase (ThermoScientific) to generate pAAV- GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232- PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5- HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV- GFP to generate pAAV- SERT.
Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5-HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Article Title: Analyzing the neuroglial brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484 , Alias: 5-HTT, AI323329 , Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Fluorescence:Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re- ligation with T4 DNA ligase (ThermoScientific) to generate pAAV- GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232- PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5- HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV- GFP to generate pAAV- SERT.
Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5-HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Article Title: Analyzing the neuroglial brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484 , Alias: 5-HTT, AI323329 , Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Recombinant:Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re- ligation with T4 DNA ligase (ThermoScientific) to generate pAAV- GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232- PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5- HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV- GFP to generate pAAV- SERT.
Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5-HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Article Title: Analyzing the neuroglial brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484 , Alias: 5-HTT, AI323329 , Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Virus:Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re- ligation with T4 DNA ligase (ThermoScientific) to generate pAAV- GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232- PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5- HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV- GFP to generate pAAV- SERT.
Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5-HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Article Title: Analyzing the neuroglial brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484 , Alias: 5-HTT, AI323329 , Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Plasmid Preparation:Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re- ligation with T4 DNA ligase (ThermoScientific) to generate pAAV- GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232- PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5- HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV- GFP to generate pAAV- SERT.
Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5-HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Article Title: Analyzing the neuroglial brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484 , Alias: 5-HTT, AI323329 , Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Expressing:Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re- ligation with T4 DNA ligase (ThermoScientific) to generate pAAV- GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232- PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5- HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV- GFP to generate pAAV- SERT.
Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5-HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Article Title: Analyzing the neuroglial brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484 , Alias: 5-HTT, AI323329 , Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Sequencing:Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re- ligation with T4 DNA ligase (ThermoScientific) to generate pAAV- GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232- PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5- HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV- GFP to generate pAAV- SERT.
Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5-HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Article Title: Analyzing the neuroglial brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484 , Alias: 5-HTT, AI323329 , Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Cloning:Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re- ligation with T4 DNA ligase (ThermoScientific) to generate pAAV- GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232- PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5- HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV- GFP to generate pAAV- SERT.
Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5-HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Article Title: Analyzing the neuroglial brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484 , Alias: 5-HTT, AI323329 , Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Transfection:Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re- ligation with T4 DNA ligase (ThermoScientific) to generate pAAV- GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232- PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5- HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV- GFP to generate pAAV- SERT.
Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5-HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Article Title: Analyzing the neuroglial brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484 , Alias: 5-HTT, AI323329 , Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Software:Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re- ligation with T4 DNA ligase (ThermoScientific) to generate pAAV- GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232- PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5- HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV- GFP to generate pAAV- SERT.
Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5-HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Article Title: Analyzing the neuroglial brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484 , Alias: 5-HTT, AI323329 , Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Activity Assay:Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re- ligation with T4 DNA ligase (ThermoScientific) to generate pAAV- GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232- PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5- HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV- GFP to generate pAAV- SERT.
Article Title: Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484, Alias: 5-HTT, AI323329, Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
Article Title: Analyzing the neuroglial brainstem circuits for respiratory chemosensitivity in freely moving mice
Article Snippet: ClaI, blunting with Klenow Fragment (TheroScientific) and re-ligation with T4 DNA ligase (ThermoScientific) to generate pAAV-GFP without promoter. .. A SERT promoter clone was purchased from Genecopoiea (Product ID: MPRM41232-PG02, Symbol: Slc6a4, Species: Mouse, Target Gene Accession: NM_010484 , Alias: 5-HTT, AI323329 , Htt, SertA). .. The promoter region was cut out with EcoRI and HndIII and introduced by T4 DNA ligation into the previously modified pAAV-GFP to generate pAAV-SERT.
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